Genetic relationship and diversity among coconut (Cocos nucifera L.) accessions revealed through SCoT analysis
نویسندگان
چکیده
Coconut (Cocos nucifera L.) is one of the important palms grown both as a homestead and plantation crop in countries and most island territories of tropical regions. Different DNA-based marker systems have been utilized to assess the extent of genetic diversity in coconut. Advances in genomics research have resulted in the development of novel gene-targeted markers. In the present study, we have used a simple and novel marker system, start codon targeted polymorphism (SCoT), for its evaluation as a potential marker system in coconut. SCoT markers were utilized for assessment of genetic diversity in 23 coconut accessions (10 talls and 13 dwarfs), representing different geographical regions. Out of 25 SCoT primers screened, 15 primers were selected for this study based on their consistent amplification patterns. A total of 102 scorable bands were produced by the 15 primers, 88 % of which were polymorphic. The scored data were used to construct a similarity matrix. The similarity coefficient values ranged between 0.37 and 0.91. These coefficients were utilized to construct a dendrogram using the unweighted pair group of arithmetic means (UPGMA). The extent of genetic diversity observed based on SCoT analysis of coconut accessions was comparable to earlier findings using other marker systems. Tall and dwarf coconut accessions were clearly demarcated, and in general, coconut accessions from the same geographical region clustered together. The results indicate the potential of SCoT markers to be utilized as molecular markers to detect DNA polymorphism in coconut accessions.
منابع مشابه
Independent Origins of Cultivated Coconut (Cocos nucifera L.) in the Old World Tropics
As a portable source of food, water, fuel, and construction materials, the coconut (Cocos nucifera L.) played a fundamental role in human migrations and the development of civilization across the humid tropics. Here we investigated the coconut's domestication history and its population genetic structure as it relates to human dispersal patterns. A sample of 1,322 coconut accessions, representin...
متن کاملUse of microsatellite DNA markers to investigate the level of genetic diversity and population genetic structure of coconut (Cocos nucifera L.).
We have used eight pairs of simple sequence repeat (SSR) primers to analyse the genetic diversity in 130 individuals of coconut (Cocos nucifera L.) comprising 75 tall individuals and 55 dwarf individuals, representing 94 different coconut ecotypes throughout the world. A total of 51 alleles were detected, with an average of 6.4 alleles per locus. Fifty alleles were detected in tall coconuts (ta...
متن کاملGenetic Fidelity Testing Using SSR Marker Assay Confirms Trueness to Type of Micropropagated Coconut (Cocos nucifera L.) Plantlets Derived from Unfertilized Ovaries
RESEARCH ARTICLE Genetic Fidelity Testing Using SSR Marker Assay Confirms Trueness to Type of Micropropagated Coconut (Cocos nucifera L.) Plantlets Derived from Unfertilized Ovaries H.D.D. Bandupriya, W.W.M.A. Iroshini, S A C N Perera, V.R.M. Vidhanaarachchi, S.C. Fernando, E.S. Santha and T.R. Gunathilake Tissue Culture Division, Coconut Research Institute, Lunuwila, Sri Lanka Department of Ex...
متن کاملAnalgesic effect of coconut shell (Cocos nucifera L) liquid smoke on mice
Background: Drugs can be used to eliminate pain by inhibiting the activity of conversing arachidonic acid into prostaglandin. The chemical compositions of coconut shell are cellulose, pentosan, lignin, solvent extraction, uronat anhydrous, nitrogen, and water. One active ingredient in coconut shell is phenyl propanoid (consisting in lignin structure) and guaicol. Phenyl propanoid and guaicol ar...
متن کاملبررسی تنوع مورفولوژیکی تودههای سیاهدانه (Nigella sativa L.) با استفاده از روشهای آماری چند متغیره
Nigella sativa L. (black cumin) belonging to the Ranunculaceae family, is one of the most important medicinal plants and wild and cultivated forms of this plant is used in Iran. Genetic diversity of 27 accessions of N .Sativa L. from different places of Iran was characterized by morphological characteristics and data was analyzed using univariate and multivariate analyses. ANOVA revealed high s...
متن کامل